Figure 8.

sdha-2 mutants display rare, spontaneous, non-heritable reversion to wild-type fertility

(A) Rarely, a sdha-2 mutant animal displayed a large outlier brood size. We screened descendants of these outliers in replicate (n = 5-8, 2-3 for controls) and could not recapitulate the large brood size. Data are mean ± SEM where applicable. Descendant and control data were collected in one assay, outlier data was collected in separate, previous assays.

(B) Gene set enrichment analysis (GSEA) of mRNA transcripts after each stress exposure. A positive normalized enrichment score (NES) indicates that the gene set was upregulated after stress exposure. Hollow triangles indicate a lack of statistical significance (FDR>0.05, -Log2(FDR) < 4.3). Gene sets were based on GO term categories and manually curated for greater accuracy, see STAR Methods for details.

(C) Heatmap of SDH complex members and immediate up and downstream pathway factors.

(D) Heatmap of all genes significantly differentially expressed in both heat shock and 4-h starvation datasets. Clustering was performed using Euclidean row distance and ward.D2 clustering method.

(E) Heatmap of known sperm-related genes found within the Class I gene set.