RNAseq stress conditions

Stress name	Method	Source
1hr–16hr Starved	L4 worms were washed onto unseeded plates and left for the indicated time.	Harvald et al.91
Heat shock	Synchronised L1 worms were placed on HT115 bacteria containing the empty L4440 plasmid. Heat shock was performed at the L4 stage for 30 minutes in a 33C water bath.	Brunquell, J. et al.92
Post-dauer Starved	Starvation-induced post-dauer adults. Worms were placed on OP50 plates and left to starve. 1% SDS was used to select for dauers which were then transferred to fresh plates fed as normal until they were day 1 adults.	Ow et al.93
Rotenone	L4 worms were washed onto heat inactivated OP50 plates with 100nm Rotenone, an ETC complex I inhibitor, and left for 24 hours.	Schmeisser et al.94
Pathogen	Embryos isolated from bleached adult hermaphrodites were raised on growth plates with L4440 bacteria until the L4/young adult transition stage, then transferred to OP50 or PA14 bacteria plates for 6 hours.	Ding et al.95