Table 3.
Viral RNAs were determined by reverse transcription polymerase chain reaction (RT-PCR) in different tissues from euthanized calves at 28 dpi.
| Tissues | No. of calves tested positive for viral | |||
|---|---|---|---|---|
| RNAs/total no. of calves tested | ||||
|
BVDV-1a
HN-03a |
BVDV-1m
HB-01 |
BVDV-1v
HB-03 |
Negative
control |
|
| Heart | 0/3 | 0/3 | 0/3 | 0/3 |
| Lung | 2/3b | 2/3 | 1/3 | 0/3 |
| Liver | 0/3 | 0/3 | 0/3 | 0/3 |
| Kidney | 0/3 | 0/3 | 0/3 | 0/3 |
| Spleen | 1/3b | 1/3 | 0/3 | 0/3 |
| Duodenum | 0/3 | 0/3 | 0/3 | 0/3 |
| Jejunum | 1/3b | 0/3 | 0/3 | 0/3 |
| Ileum | 1/3b | 1/3 | 0/3 | 0/3 |
| Tonsil | 2/3b | 1/3 | 1/3 | 0/3 |
| Superficial cervical | 2/3b | 2/3 | 1/3 | 0/3 |
| lymph node | ||||
| Mesenteric | 1/3 | 1/3 | 0/3 | 0/3 |
| lymph node | ||||
| Blood | 0/3 | 0/3 | 0/3 | 0/3 |
a
Means one calf died naturally at 21dpi.
b
Means the sample of calf died at 21 dpi was tested positive.