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. 2022 Nov 16;13:1012516. doi: 10.3389/fmicb.2022.1012516

Figure 2.

Figure 2

(A) Time-kill curve. The tested strain was Cryptococcus neoformans H99. The concentrations of allicin were NC (0), 0.5 MIC (1 μg/ml), 1 MIC (2 μg/ml), 2 MIC (4 μg/ml), 4 MIC (8 μg/ml) and 8 MIC (16 μg/ml). The colonies were counted after incubation for 48 h. (B) Growth time and division of C. neoformans H99. Cryptococcus continued to grow in the control group, allicin at 1 MIC significantly inhibited growth, allicin at 2 MIC and 4 MIC significantly prolonged the division of Cryptococcus within 1 h, and no division was observed after incubation for 2 h. (C) Real-time imaging of living C. neoformans H99. Continuous photographs were obtained to observe the overall growth of C. neoformans H99 after long-term culture in the presence of allicin (left). The cells were incubated for 24, 48, 72, and 96 h, and the long-term growth changes in Cryptococcus were observed. (D) The inhibitory effect of allicin on the proliferation and division of Cryptococcus (right) was observed by continuous photographs at 2, 3, 4, and 5 h.