Table 2.

Alpha diversity by Shannon index and Richness.

	Alpha diversity		PERMANOVA Individual variables		PERMANOVA all variables		PERMANOVA select variables		sPLS-DA
Variable	Richness	Shannon	R2 (%)	P (>F)	R2 (%)	P (>F)	R2 (%)	P (>F)	AUC	CCR (%)
Smoking	0.3498	2.185x10-0.5	3.254	0.001	3.020	0.001	2.937	0.001	0.95	79.6
Alcohol intake	0.02736	0.04786	1.066	0.001	0.605	0.01	0.649	0.01	0.66 0.61 0.64	51.6
Glycemic status category	0.006883	0.08179	0.435	0.043	0.339	0.37	0.482	0.08	0.69	55.8
Sex	0.09062	0.995	0.334	0.01	0.270	0.08	0.290	0.04	0.73	51.3
HbA1c	0.0138	0.2477	0.698	0.002	0.408	0.15	0.440	0.12	0.66	83.9
Activity level	0.2949	0.1229	0.423	0.07	0.366	0.25	0.405	0.17	-	-
BMI	0.1654	0.8998	0.506	0.12	0.593	0.18	-	-	-	-
Blood pressure	0.148	0.2339	0.413	0.39	0.487	0.41	-	-	-	-
Systolic blood pressure	0.7762	0.2679	0.474	0.21	0.604	0.12	-	-	-	-
Diastolic blood pressure	0.5455	0.2382	0.563	0.08	0.464	0.48	-	-	-	-
Heart rate	0.3565	0.5538	0.377	0.10	0.301	0.50	-	-	-	-
Pulse pressure	0.7721	0.8423	0.091	0.77	0.258	0.08	-	-	-	-
Triglycerides	0.6569	0.07657	0.231	0.08	0.236	0.13	-	-	-	-
HDL cholesterol	0.7306	0.2483	0.203	0.12	0.085	0.91	-	-	-	-
LDL cholesterol	0.02109	0.03238	0.195	0.12	0.212	0,21	-	-	-	-
Total cholesterol	0.02435	0.2011	0.139	0.38	0.119	0,69	-	-	-	-
Waist	0.8994	0.6909	0.246	0.54	0.260	0,69	-	-	-	-
Waist to height ratio	0.2941	0.3842	0.189	0.15	0.188	0.27	-	-	-	-

PERMANOVA results for all variables individual and together. AUC and correct classification rate of predictive models using sPLSDA.