Fig. 2. Effects of IFN-γ and different inhibitors on the proportion of lymphocytes in BALF and White Blood Cells, and the F-actin level in lung-resident lymphocytes. (A) Summarized data showing the proportion of lymphocytes in BALF are representative of three independent experiments. Mice in different groups were treated as follows: the control group (Intranasally instilled with PBS, n = 9), low IFN-γ group (intranasally instilled with 3.125 µg/kg IFN-γ, n = 9), medium IFN-γ group (intranasally instilled with 12.5 µg/kg IFN-γ, n = 10) and high IFN-γ group (intranasally instilled with 50 µg/kg IFN-γ, n = 10). (B) Group data showing the effects of IFN-γ instillation and different inhibitors on the proportion of lymphocytes in BALF (n = 5 per group). (C) Group data showing the effects of IFN-γ instillation and different inhibitors on the proportion of lymphocytes in White Blood Cells (n = 5 per group). (D) Summarized data representing the fluorescence value of F-actins in lung-resident lymphocytes (n = 5 per group). Mice in different groups were treated as the same as that in Fig. 1. Data are shown as mean ± SD.

BALF, bronchoalveolar lavage fluid; IFN-γ, interferon-γ; CXCR3, CXC chemokine receptor 3; ERK, extracellular signal-regulated kinase.

^*P < 0.05, compared with the “Control”; ^†P < 0.05, compared with the “IFN-γ model.”