Table 1.
List of genetic crosses, embryo numbers analyzed, and experimental outcomes related to each figure.
| Alleles crossed | shorthand notation | %rescue†/analysis | Tam Tx* | phenotype/analysis | Fig. |
|---|---|---|---|---|---|
|
| |||||
|
Shh+/C;Bax+/C;Bak−/−;Hoxb6CreER x ShhC/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh-CKO;Bax+/c |
18/31 (Bax-KO); 0/28 (Bax-het) |
E9.5+3h | skeleton | 1 |
|
| |||||
|
Shh+/−;Bax+/C;Bak−/−;Hoxb6CreER x Shh+/−;Bax C/C;Bak−/− |
Shh−/−;Bax-CKO | 0/18 (Bax-KO) | E8.75–E9.5 | skeleton | 1 |
|
| |||||
|
Shh+/−;Bax
+/−;Bak−/− x Shh+/−;Bax +/−;Bak−/− |
Shh−/−;Bax−/− | 0/6 (Bax/Bak-KO) | NA | skeleton | 1 |
|
| |||||
|
Shh+/C;BaxC/C;Bak−/−;Hoxb6CreER x ShhC/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO | NA-see Figs. 1, S1, text | E9.5+3h | Shh activity duration |
1, S1 |
|
| |||||
|
Shh+/−;Bax+/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh−/−;Bax-CKO |
NA-see Fig. S1, text | E9.5+3h | Cell survival | S1 |
|
| |||||
|
ShhCreER/+;RosaLacZ/LacZ x Gli1CreER/+;RosaLacZ/LacZ |
ShhCreER+ or Gli1CreER+ |
-51 embryos -60 embryos |
Varied-Fig 2 | Shh-expression, -response lineage | 2 |
|
| |||||
|
Shh+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh−/−;Bax-CKO |
NA-see Figs. 3, S2 text | E9.5+3h | In situ HCR – Shh, Gli1 (response) |
3, S2 |
|
| |||||
|
Shh+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh−/−;Bax-CKO |
NA-see Fig. 4, text | E9.5+3h | Hand2, Ptch1, Gli1 Gli3 western | 4 |
|
| |||||
|
Shh+/−;Gli3+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh+/−;Bax C/C;Bak−/−;Hoxb6CreER |
Shh−/−;Gli3+/−;BaxCKO | 10/10 (vs Shh−/−) | NA or E9.5+3h | skeleton - Gli3 dose modulation | 4 |
| Shh+/−;Gli3+/− x Shh+/− | Shh−/−;Gli3+/− | 8/8 (vs Shh−/−) | |||
| Shh+/−;Gli3+/− x ShhC/C;Hoxb6CreER | Shh-CKO;Gli3+/− | 12/12 (vs Shh-CKO) | |||
|
Shh+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO | 18/31 (vs Shh-CKO) | |||
|
| |||||
|
Shh+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh−/−;Bax-CKO |
NA-see Fig. 5, text | E9.5+3h | Fgf8, Grem1, Hoxa13, Hoxd13 | 5 |
|
| |||||
|
Shh+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh−/−;Bax-CKO |
NA-see Fig. S3, text | E9.5+3h | Jag1, Cyp26b1, Hoxd11, Hoxd13 | S3 |
|
| |||||
|
Shh+/−;BaxC/C;Bak−/−;Hoxb6CreER x Shh−/C;Bax C/C;Bak−/−;Hoxb6CreER |
Shh-CKO;Bax-CKO Shh−/−;Bax-CKO |
NA-see Fig. S4, text | E9.5+3h | Anterior pattern genes: Alx4, Irx3 | S4 |
|
| |||||
|
ShhCre/+;RosaLacZ/LacZ x Shh+/−;RosaSmoM2/SmoM2 |
ShhCre/−;Shh-SmoM2 | 21/32 (digit 1+) | NA | Skeleton; Hoxa13, Shh-lineage | 6 |
| ShhCre/+;RosaLacZ/LacZ x Shh+/− | ShhCre/− | 0/6 (digit 1+) | |||
|
| |||||
|
ShhCre/+;Bax+/−;Bak−/−;RosaLacZ/LacZ x Shh+/− ;Bax +/−;Bak−/−;RosaSmoM2/ SmoM2 |
ShhCre/−;Bax/Bak-KO; Shh-SmoM2 |
8/8 (only digit 1+) | NA | skeleton | 6 |
|
| |||||
|
ShhCre/+;RosaLacZ/LacZ x Shh+/−;RosaSmoM2/+ |
ShhCre/+;Shh-SmoM2 | 4/64 (ectopic ZPA, duplicated d1) | NA | skeleton Shh-lineage | S5 |
| ShhCre/−;Shh-SmoM2 ShhCre/− |
NA-see Fig. S5, text | Uncx4.1, Sox9 Ptch1, Gli1 | |||
|
| |||||
|
ShhCre/+;RosaLacZ/LacZ x Shh+/−;RosaSmoM2/+ ShhCre/+;RosaEYFP/EYFP x Shh+/−;RosaSmoM2/+ |
ShhCre/−;Shh-SmoM2 ShhCre/− |
−10 embryos − 6 embryos |
NA | Shh-lineage; cell survival | S5 |
†
skeletal numbers refer to embryo numbers (left+right hindlimb both rescued);
*
Tx, treatment time