TABLE 4.
rpoS-dependent transcriptional activity of E. coli sodC promotera
| E. coli strain | β-Gal activity (U) at:
|
||
|---|---|---|---|
| 3 h | 24 h | 48 h | |
| GC4468(pMC1403) | 0.40 ± 0.27 | 0.2 ± 0.17 | 0.1 ± 0.2 |
| GC4468(pMCPromEcSOD) | 58.75 ± 18.50 | 258.7 ± 63.3 | 420 ± 32.6 |
| QC1110(pMC1403) | 0.1 ± 0.05 | 0.11 ± 0.1 | 0.14 ± 0.03 |
| QC1110(pMCPromEcSOD) | 3.05 ± 0.4 | 31.5 ± 7.54 | 39.4 ± 1.8 |
Overnight E. coli cultures were diluted 1:100 in LB medium and grown up to 2 days at 37°C. Samples were withdrawn at different times, and activity values were standardized to cell concentrations. Cells collected at 3 h after subculturing were still in mid-log phase. The values reported here are the means ± standard deviations of four different experiments.