FIG. 1.
Reactivity of sera from DNA-vaccinated mice with TLA. Western blots of total parasite lysate were developed with a pool of 10 antisera from GRA1 DNA-vaccinated mice (lane 6, C57BL/6; lane 7, BALB/c; lane 8, C3H), GRA7 DNA-vaccinated mice (lane 9, C57BL/6; lane 10, BALB/c; lane 11, C3H), and ROP2 DNA-vaccinated mice (lane 12, C57BL/6; lane 13, BALB/c; lane 14, C3H). Reactivity from mice receiving empty vector DNA is shown in lanes 2, 3, and 4 (C57BL/6, BALB/c, and C3H, respectively). As controls, an anti-GRA1 monoclonal antibody (lane 5) and a pool from T. gondii chronically infected Swiss mice (lane 1) were included. The positions and molecular masses (in kilodaltons) of protein standards are also shown.