Seroconversion rates of C57BL/6, BALB/c, and C3H mice (serum samples from individual animals) 3 weeks after 1 (white bars), 2 (grey bars), and 3 (black bars) intramuscular injections of 100 μg of plasmid DNA encoding GRA1, GRA7, or ROP2, with a 3-week interval between two DNA administrations. Serum samples were considered positive when their absorbance values in ELISA exceeded at least by a factor of 2 the absorbance from a pool of preimmune sera at a dilution 1/200 or further. Numbers above the bars show the absolute numbers of positive/tested animals.