| Lethal impact on beneficial insects |
Non-targeted pest sharing common key genes/similar dsRNA sequence homology with the target pest |
83
|
| Lack of uniform susceptibility between species |
Enzymatic degradation of dsRNA, exposure dosage, impaired RNAi machinery, presence of virus in the target pest |
84
|
| Variation in RNAi responses within the same insect |
Insufficient spread of RNAi response throughout the body, variation in pH among different organs |
85 and 87
|
| High production costs |
High cost of chemicals, equipment, and maintenance conditions |
1
|
| Extracellular degradation of dsRNA |
Degradation by nucleases present in the digestive system |
91 and 92
|
