Skip to main content
. 2022 Sep 13;34(12):4936–4949. doi: 10.1093/plcell/koac281

Figure 7.

Figure 7

His172 is the catalytic residue necessary for proenzyme VyPAL2 activation. A, Schematic sequence representations of VyPAL2-H172A and VyPAL2-N67A/C214A. B, The products from low pH processing of the VyPAL2-H172A and VyPAL2-N67A/C214A proenzymes were analyzed using SDS–PAGE with Coomassie blue staining. Incubation of VyPAL2-H172A for up to 2 h did not lead to any processed enzyme. C, Proposed mechanism of the processing of VyPAL2 that is Cys214 independent and where His172 is the main catalytic residue. This mechanism involves the formation of an acyl-imidazole intermediate, which is highly unstable and rapidly resolved by the attack of a nucleophilic water molecule.