Figure 2.
Expression profile and localization pattern of BC16. A, RT-qPCR analysis of BC16 transcripts in the indicated tissues of the WT (Nipponbare), showing the relative level to rice HNR. S1–S8 indicate internode segments cut from the base to the tip of the developing 2nd internode. Data represent the mean ± sd of three biological replicates. R, root; L, leaf; LS, leaf sheath; P, panicle. B, RT-qPCR analysis of BC16 transcripts in fiber cells (FC) and parenchyma cells (PC) collected by laser microdissection from internode sections (left panel). Data represent the mean ± sd of four biological replicates. Rice HNR was used as an internal control. The expression level in FC was set as 1. Bar = 100 μm. C and D, Confocal images and intensity profile plots (the dashed lines) of tobacco epidermal cells coexpressing BC16-GFP together with a mCherry-tagged ER marker (HDEL) or Golgi marker (Man49). Bars = 5 μm. E and F, Confocal images and intensity profile plots (the dashed lines) of rice protoplast cells coexpressing BC16-GFP together with an ER marker (HDEL) or a Golgi marker (Man49). Bars = 5 μm. G and H, Measurement of the degree of colocalization of the GFP-BC16 fusion with ER (HDEL) and Golgi (Man49) markers in tobacco epidermal cells (G) and rice protoplasts (H) using the Pearson correlation coefficient and Mander’s coefficient. The arrow schemes above the bars indicate the intensity overlap of HDEL/Man49 with BC16 and the reversals, according to the arrow directions. Data represent the mean ± sd of 10 leaf cells or 5 protoplast cells.