Figure 4.

CUC2 and CUC3 are required for delayed CaAM development in dpa4-2 sod7-2 mutants. A–J, Inflorescences of WT, single cuc mutants, the dpa4-2 sod7-2 double mutant, and the dpa4-2 sod7-2 cuc triple mutants. Plants were grown for 5 weeks in LD. White arrowheads point to the developed CaAMs, while the arrows point to delayed CaAMs. The time point after bolting at which half of the CaAMs are developed (t₅₀, in days) is indicated under the genotype. K–N, Kinetics of CaAM development in WT (K–N), dpa4-2 sod7-2 (K–N), cuc1-13, and dpa4-2 sod7-2 cuc1-13 (K), cuc1 and dpa4-2 sod7-2 cuc1 (L), cuc3 and dpa4-2 sod7-2 cuc3 (M) and cuc3-105 and dpa4-2 sod7-2 cuc3-105 (N) plants after bolting. Development of the CaAM is indicated as the percentage of developed branches (≥3 mm) to the total number of cauline leaves (n ≥ 7). All data were generated in the same experiments, and therefore the same WT and dpa4-2 sod7-2 data were used in (K–N). Scale bars = 5 cm.