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. 2022 Aug 27;34(12):4738–4759. doi: 10.1093/plcell/koac269

Figure 6.

Figure 6

Disruption of putative NGAL binding sites in CUC3 induces ectopic CUC3 expression and a delay in CaAM development. A–E, Inflorescences of WT, cuc3-105, dpa4-2 sod7-2, pCUC3:CUC3 cuc3-105 line #1, and pCUC3-6m:CUC3-1m cuc3-105 line #13. Plants were grown for 4 weeks in SD and then shifted to LD for 3 weeks. White arrowheads point to the developed CaAMs, while the arrows point to delayed CaAMs. F, Secondary cauline stem length as a function of primary length stem for WT, cuc3-105, dpa4-2 sod7-2, two pCUC3:CUC3 cuc3-105 lines (#1 and #25), and two pCUC3-6m:CUC3-1m cuc3-105 lines (#13 and #14). G, Quantification of the transcript level of CUC3 by RT-qPCR on 10 day-old seedlings of WT, cuc3-105, dpa4-2 sod7-2, two pCUC3:CUC3 cuc3-105 lines (#1 and #25) and two pCUC3-6m:CUC3-1m cuc3-105 lines (#13 and #14). Error bars are se. A Student’s t test was performed to compare the expression levels in the mutants compared to the WT (*P < 0.05; **P < 0.01; ***P < 0.001). H, I, K, and L, Maximum projections of transverse optical sections of CaAMs of WT plants harboring the pCUC3:mCherry-N7 (H and I) or pCUC3-6m:GFP-N7 (K and L) reporters during CaAM formation at the eye (H and K) and (I and L) dome stage. Orthoslices through the developing AM are shown below the transverse sections. J and M, Mean fluorescence along the radial axis of CaAMs harboring the pCUC3:mCherry-N7 (L) or pCUC3-6m:GFP-N7 (M) reporters at the dome stage. (n ≥ 5). Scale bars: (A–E)  = 5 cm; (H, I, K, and L)   = 50 µm. clp: cauline leaf primordium; asterisks: AM; the dotted lines correspond to the edges of the cauline leaf primordia.