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. 2022 Nov 30;24:85. doi: 10.1186/s13058-022-01583-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Set of q3D-HDMI processing steps after image formation and segmentation to prepare the image for quantification. a Microvasculature image of malignant breast tumor obtained from Amira 3D, b binary microvasculature image generated in MATLAB, c skeleton showing the network of vessels, d the skeleton overlaid on the binary vessel image. HDMI = high-definition microvasculature imaging