Figure 4.

Autologously reconstituted human carcinoma is infiltrated with endogenous human hematopoietic lymphocytes that mature in the thymus. CD34+ BMC were transduced with lentivirus vectors containing GFP protein prior to BMC engraftment. Autologously reconstituted tumor was harvested, stained with DAPI, and immunofluorescent microscopy performed. The left panels show tumor with only DAPI counterstain. The left upper panel is tumor xenografted into non-humanized mice, while the left lower panel is the tumor in autologously humanized mice (white arrowhead – lymphocyte; white arrow – myeloid cells). Slides were co-stained with human CD3 and CD11b conjugates (right panels). The bar scale is 100 μ (from Fu, et.al., 2016).