Skip to main content
. Author manuscript; available in PMC: 2022 Nov 30.
Published in final edited form as: J Immunol. 2019 Jun 21;203(3):627–638. doi: 10.4049/jimmunol.1900055

Figure 5. NKT cell subset development in the absence of YY1.

Figure 5.

FACs analysis was used to determine the expression of key transcription factors associated with NKT cell effector functions in YY1 deficient thymus NKT cells. NKT cells isolated from the thymuses of C57BL/6 (WT) mice and PLZF-Cre YY1 flx.flx mice were stained for the transcription factors PLZF, Tbet and ROR γT. Gating schemes and representative FACS data for the (A) NKT1, NKT2, and (B) NKT17 populations are shown. (C) The frequency and absolute number of thymic WT NKT cells and thymic YY1− cKO NKT cells in these subpopulations are summarized. Data are from 4 or more independent experiments representing 4 biological replicates. The horizontal lines indicate the mean (±s.e.m.). *P<0.05determined by Mann Whitney U-Test (C).