FACs analysis was used to determine the expression of key transcription factors associated with NKT cell effector functions in YY1 deficient spleen NKT cells. Splenic NKT cells isolated from WT and PLZF-Cre YY1 flx.flx mice were stained for PLZF, Tbet, and ROR γT (A, B). The frequency and absolute number of splenic WT NKT cells and YY1 deficient NKT cells present in the NKT1, NKT2, and NKT17 subpopulations are summarized in (C). Data are from 4 or more independent experiments representing 4 biological replicates. The horizontal lines indicate the mean (±s.e.m.). *P<0.05, **P<0.01, determined by Mann Whitney U-Test (C).