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. 2022 Nov 7;18(6):1672–1677. doi: 10.5114/aoms/154956

Figure 3.

Figure 3

A – Detection of ajuba protein expression in BMSC during lentiviral infection and differentiation of mir-21-OE and mir-21-KD, a – WB was used to detect the expression changes of ajuba in 1 week, 2 weeks and 3 weeks after miR-21 over-expression, b – After the decrease of miR-21 was detected by WB. B – Detection of Isl1 protein expression in BMSC during lentiviral infection and differentiation of mir-21-OE and mir-21-KD, a – WB was used to detect the expression of Isl1 in 1 week, 2 weeks and 3 weeks after miR-21 overexpression, b – WB was used to detect the changes of Isl1 expression in 1 week, 2 weeks and 3 weeks after the decrease of miR-21. C – Detection of ajuba, Isl1 and cTnI protein expression in BMSC after ajuba overexpression and ajuba interference, a – WB was used to detect the expression changes of ajuba, Isl1 and cTnI proteins after ajuba overexpression, b – WB was used to detect the expression changes of ajuba, Isl1 and cTnI proteins after ajuba interference knockdown. D – After ajuba overexpression, the expression of cTnI protein was detectedby immunofluorescence. E – The expression of cTnI protein in ajuba interference knockdown group and control group was detected by immunofluorescence. The photo magnification is 200×