Table 1.

Experimental protocol.

Day 1	Arrive in lab at 0900 PVT assessed at 1115, 1415, 1715, 2015, and 2315 Sleepiness and mood assessed at 1120, 1420, 1720, 2020, and 2320 Pain threshold test at 1130, 1430, 1730, 2030, 2330
Day 2	Z-machine recorded sleep at 0000-0800 Ultra-short sleep-wake cycle 0800-2400 Collection of every urine voiding for aMT6s6 Forehead temperature assessed at 0810, 0950, 1110, 1250, 1410, 1550, 1710, 1850, 2010, 2150, 2310 PVT assessed at 0815, 1115, 1415, 1715, 2015, 2315 Mood and sleepiness assessed at 0820, 1120, 1420, 1720, 2020, 2320 Pain senstitivy at 0830, 1130, 1430, 1730, 2030, 2330 Wingate Test at 0900, 1500, and 2100
Day 3	Ultra-short sleep wake cycle from 0000-1000 Every urine voiding collected for aMT6s from 0000-1000 Forehead temperature at 0050, 0210, 0350, 0510. 0650, 0810, 0950 PVT assessed at 0215, 0515, 0815, 1115, 1415, 1715, 2015, 2315 Mood and sleepiness assessed at 0220, 0520, 0820, 1120, 1420, 1720, 2020, 2320 Pain sensitivity at 0230, 0530, 0830 Wingate Trialsat 0300 Melatonin capsule at 1400 Exercise at 2130-2230
Day 4	Bright Light 0130-0230 PVT assessed at 0215, 0515, 1715, 2015, 2315 Sleepiness and mood assessed at 0220, 0520, 1720, 2020, 2320 Pain sensitivity at 1730, 2030, 2330 Z-machine recorded sleep 0800-1600 Melatonin capsule at 1630
Day 5	Exercise at 0000-0100 PVT assessed at 0215, 0515, 1715, 2015, 2315 Bright Light 0400-0500 Sleepiness and mood assessed at 0220, 0520, 1720, 2020, 2320 Pain threshold at 0230, 0530, 1730, 2030, 2330 Z-machine recorded sleep at 0800-1600 Melatonin Capsule at 1900
Day 6	PVT assessed at 0215, 0515, 1715, 2015, 2315 Sleepiness and mood assessed at 0220, 0520, 1720, 2020, 2320 Exercise at 0230-0330 Pain sensitivity at 0530, 1730, 2030, 2330 Bright Light at 0630-0730 Z-machine recorded sleep 0800-1600 Ultrashort sleep wake cycle from 1600-2400 Every urine voiding collected from 1600-2400 for aMT6s Forehead tempeature 1610, 1750, 1910, 2050, 2210, 2350 Wingate test at either 1700 and 2300 or 2000, or 2300
Day 7	Ultra-short sleep wake cycle from 0000-1800 Every urine voliding collected for aMT6s Forehead tempeature at 0150, 0310, 0450, 0610, 0750, 0910, 1050, 1210, 1350, 1510, 1650 Wingate test at 0500 and 1100 PVT assessed at 0215, 0515, 0815, 1115, 1415, 1715 Mood and sleepiness assessed at 0220, 0520, 0820, 1120, 1420, 1720 Pain sensitivity at 0230, 0530, 0830

Times below are for baseline sleep of 0000-0800 h, and the Bright light + Exercise + Melatonin treatment on days 3-5. Four trials of the Wingate test will each be separated by 6 hours. The timing of the trials will be staggered with subjects performing the first trial at 0900, 1200, or 1500. Please note: All the times noted are the Arizona time, including after the 8 h delay of the sleep-wake and light dark schedul (starting on day 3). The example is provided for a subject with a baseline sleep schedule of 0000-0800 who was assigned to complete the first Wingat Trial at 0900 on Day 2.