FIG. 2.

Northern blot analysis of transfectants for gp63 sense and antisense transcripts. Total RNA samples from different transfectants are shown. Lane 1, P6.5/1.9; lanes 2 and 3, P6.5/1.9R; lane 4, P6.5 alone. All transfectants were grown in 20 μg of TM/ml, except the sample from P6.5/1.9R in lane 2, which was grown in drug-free medium. Probes used are oligonucleotides specific to sense gp63 transcripts (A), oligonucleotides for antisense gp63 transcripts (B), and the beta-tubulin (β-tub) gene of L. amazonensis (C).