Fig 4. Testing of DB with orthologous receptors in dogs and humans with two different recombinant cell lines.

In addition to the Gα16i/o44 cell line, the Gα16/gust44 cell line was used. Results were similar with concentration-dependent responses only obtained with dTas2r4. When using the Gα16/gust44 cell line, small responses were observed with the highest concentration of DB only (10mM) for dTas2r10 and dTas2r43. These responses were significant (Student’s t-test, p <0.001). Compound concentration is plotted on a log scale on the x-axis, the y-axis shows the maximum change in fluorescence divided by the baseline fluorescence before compound injection (ΔF/F₀). Data for dTas2r4 is taken from the confirmatory screening and thus appears in S2 Fig also.