Table 3. Decreasing Wapl gene dosage does not rescue lethality in Nipbl+/− mice, and decreasing Nipbl gene dosage does not rescue lethality in WaplΔ/Flox mice.
Genotypes of 58 weanlings were determined. Expected1, numbers of progeny expected assuming that all genotypes survive; χ2 = 79.228 with 3 df, P < 0.0001. Expected2, numbers of progeny expected assuming that Wapl haploinsufficiency fully rescues lethality in Nipbl+/− mice; χ2 = 39.671 with 2 df, P < 0.0001. Expected3, numbers of progeny assuming that Wapl haploinsufficiency cannot rescue lethality in Nipbl+/− mice; χ2 = 0.114 with 1 df, P = 0.7357.
| Cross: WaplFlox/+; Nipbl+/+ x WaplΔ/+; NipblFlox/+ Tg(Stra8-icre) | ||||||||
| Genotypes |
Wapl
Flox/Δ
Nipbl +/+ |
Wapl
+/Δ
Nipbl +/+ |
Wapl
Flox/+
Nipbl +/+ |
Wapl
Flox/+
Nipbl +/+ |
Wapl
Flox/Δ
Nipbl +/− |
Wapl
+/Δ
Nipbl +/− |
Wapl
+/Δ
Nipbl +/− |
Wapl
+/Δ
Nipbl +/− |
| Observed | 0 | 19 | 20 | 19 | 0 | 0 | 0 | 0 |
| Expected1 | 7.25 | 7.25 | 7.25 | 7.25 | 7.25 | 7.25 | 7.25 | 7.25 |
| Expected2 | 0 | 11.6 | 11.6 | 11.6 | 11.6 | 11.6 | 0 | 0 |
| Expected3 | 0 | 19.33 | 19.33 | 19.33 | 0 | 0 | 0 | 0 |