TABLE 1.
Cytokine analysisa of culture supernatants of murine lymph node cells after in vitro stimulation with PfLSA-1-derived synthetic peptides
| Peptide | Peptide concn (μg/ml) | Secretion of
indicated cytokine (pg/ml) by:
|
|||||
|---|---|---|---|---|---|---|---|
| BALB/c
mice
|
C57BL/6 mice
|
||||||
| IL-2 | IFN-γ | IL-4 | IL-2 | IFN-γ | IL-4 | ||
| LS1.1 | 0 | <5 | 75 | <5 | 40 | <10 | <5 |
| 10 | 120 | 180 | <5 | 360 | 9,800 | <5 | |
| 20 | 120 | 360 | <5 | 260 | 5,600 | <5 | |
| 40 | 260 | 1,500 | <5 | 280 | 1,200 | <5 | |
| LS1.2 | 0 | 40 | 100 | <5 | 80 | 60 | <5 |
| 10 | 160 | 800 | <5 | 400 | 10,400 | <5 | |
| 20 | 180 | 660 | <5 | 360 | 8,800 | <5 | |
| 40 | 90 | 160 | <5 | 440 | 9,200 | <5 | |
| LS1.3 | 0 | 70 | 120 | <5 | 15 | <10 | <5 |
| 10 | 360 | 600 | <5 | 18 | 135 | <5 | |
| 20 | 180 | 3,000 | <5 | 30 | 540 | <5 | |
| 40 | 135 | 10,200 | <5 | 30 | 675 | <5 | |
| LS1.4 | 0 | 50 | 120 | <5 | 8 | <10 | <5 |
| 10 | 180 | 450 | <5 | 18 | 1,440 | <5 | |
| 20 | 165 | 600 | <5 | 30 | 600 | <5 | |
| 40 | 135 | 1,560 | <5 | 8 | 510 | <5 | |
| LS1.5 | 0 | 60 | <10 | <5 | 10 | <10 | <5 |
| 10 | 165 | 120 | <5 | 15 | <10 | <5 | |
| 20 | 225 | 180 | <5 | 18 | 45 | <5 | |
| 40 | 165 | 45 | <5 | 15 | 30 | <5 | |
| LS1.6 | 0 | <5 | <10 | <5 | <5 | <10 | <5 |
| 10 | <5 | <10 | <5 | <5 | <10 | <5 | |
| 20 | <5 | <10 | <5 | <5 | <10 | <5 | |
| 40 | <5 | <10 | <5 | <5 | <10 | <5 | |
| LS1.7 | 0 | <5 | <10 | <5 | <5 | <10 | <5 |
| 10 | <5 | <10 | <5 | <5 | <10 | <5 | |
| 20 | <5 | <10 | <5 | <5 | <10 | <5 | |
| 40 | <5 | <10 | <5 | <5 | <10 | <5 | |
| LS1R | 0 | <5 | <10 | <5 | <5 | <10 | <5 |
| 10 | 45 | 525 | 75 | <5 | <10 | <5 | |
| 20 | 60 | 705 | 85 | <5 | <10 | <5 | |
| 40 | 135 | 840 | 240 | <5 | <10 | <5 | |
Cytokine analysis was performed by using specific murine cytokine ELISA kits. Data analysis was done with the respective standard cytokine curves.