TABLE 2.
Lymphoproliferation and IFN-γ secretion by naive and P. berghei-immune T. gazellae splenocytes upon in vitro stimulation with PfLSA-1-derived peptides
| Stimulanta | SI for indicated
lymphocytes
|
IFN-γ secretion (U/ml)b | |
|---|---|---|---|
| Naive | Immune | ||
| LS1.1 | 0.6 | 1.2 | <5 |
| LS1.2 | 1.3 | 4.3 | 28 |
| LS1.3 | 0.8 | 1.2 | <5 |
| LS1.4 | 0.75 | 0.8 | <5 |
| LS1.5 | 0.6 | 0.75 | <5 |
| LS1.6 | 0.86 | 0.7 | <5 |
| LS1.7 | 1.4 | 0.75 | <5 |
| LS1R | 0.9 | 1.1 | 12 |
a
Splenocytes were stimulated with various peptides at the final concentration of 20 μg/ml.
b
IFN-γ analysis was done only with culture supernatants of P. berghei-immune splenocytes by using a rat IFN-γ ELISA kit. The IFN-γ concentration in the control culture (no antigen) supernatant was always <5 U/ml.