TABLE 3.
Reactivities of H. pylori LPS with sera from humans with H. pylori infection and MAbs against Lewis antigens
| Strain from which LPS was obtained | Reactivity with human sera
|
Reactivity with anti-Lewis antigen MAbs by IB
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Type A
|
Type B
|
Type C
|
Lex | Ley | Lea | Leb | H1 (Led) | |||||
| IBa | ELISAb | IB | ELISA | IB | ELISA
|
|||||||
| Serum 1 | Serum 2 | |||||||||||
| GU2 | + | 51,200 | − | 1,600 | + | 102,400 | 51,200 | + | + | + | − | − |
| DU1 | + | 51,200 | − | 1,600 | + | 102,400 | 51,200 | − | + | − | + | − |
| CA1 | + | 12,800 | − | 800 | + | 1,600 | 3,200 | − | + | − | − | − |
| CA2 | − | 3,200 | + | 12,800 | + | 12,800 | 12,800 | − | + | − | − | − |
| CA4 | − | 3,200 | + | 6,400 | + | 6,400 | 3,200 | + | − | + | − | − |
| CA5 | − | 1,600 | + | 12,800 | + | 12,800 | 6,400 | + | + | − | − | − |
| CA6 | − | 1,600 | + | 12,800 | + | 6,400 | 3,200 | − | − | + | − | − |
| CA9 | − | 1,600 | + | 12,800 | + | 12,800 | 6,400 | − | − | − | − | + |
| CG10 | − | 1,600 | − | 400 | − | 1,600 | 800 | − | − | − | − | − |
| DU8 | − | 1,600 | − | 400 | − | 1,600 | 800 | − | − | − | − | − |
a
IB, immunoblotting.
b
The maximal serum dilution giving an A450 of 0.2 in ELISA was expressed as the serum titer. Type A serum no. 2 (from a CA patient), type B serum no. 2 (from a healthy carrier), and type C serum no. 1 (from a CG patient) and 2 (from a CA patient) were used for the assays whose results are shown from the left to the right, respectively.