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. 2022 Nov 22;11:e83743. doi: 10.7554/eLife.83743

Figure 6. Hypothetic model for NOX2 activation.

(A) Resting state of the phagocyte NADPH oxidase. NOX2, p22, and cytosolic factors are shown as cartoon and colored the same as Figure 1A. (B) Activated state of the phagocyte NADPH oxidase. The electron transfer pathway is indicted with gray arrows.

Figure 6.

Figure 6—figure supplement 1. Distinct interaction mode between NOX2-p22 complex and DUOX1-DUOXA1 complex.

Figure 6—figure supplement 1.

(A) Structure of human NOX2 and p22 complex (PDB ID: 8GZ3) in cartoon representation. The colors of each individual domain are the same as in Figure 1I. The approximate boundaries of the phospholipid bilayer are indicated as gray thick lines. Sugar moieties, haems, FAD, and lipids are shown as gold, purple, pink, and aquamarine sticks, respectively. (B) Structure of human DUOX1-DUOXA1 complex (PDB ID: 7D3F) in cartoon representation. PHD: peroxidase homology domain of DUOX1; PHLD: pleckstrin homology-like domain of DUOX1; EF: EF-hand calcium-binding module of DUOX1; DH: dehydrogenase domain of DUOX1; CLD: claudin-like domain of DUOXA1. The M0 helix, PHLD, and EF are colored in orange, and the other part of DUOX1 is colored in blue. The DUOXA1 is colored in gray. (C) Top view of the cross-section of NOX2-p22 cryo-EM map on the transmembrane layer at the position indicated as a dashed line in A. For clarity, the cryo-EM map was low-pass filtered to 7 Å. (D) Top view of the cross-section of DUOX1-DUOXA1 cryo-EM map on the transmembrane layer at the position indicated as a dashed line in B.

Figure 6—figure supplement 2. Structural comparison between NOX2-7D5 complex and NOX2-7G5 complex.

Figure 6—figure supplement 2.

(A) Overall structural comparison of human NOX2-7D5 complex between this study (orange and blue, PDB ID: 8GZ3) and NOX2-7G5 complex (pink, PDB ID: 7U8G). (B) Structure of NOX2-7D5 complex in cartoon representation. The colors of each individual domain are the same as in Figure 1I. The residues participate the interaction between NOX2 and 7D5 are colored in yellow. (C) Structure of NOX2-7G5 complex colored the same as in B (PDB ID: 7U8G).