Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Homo sapiens) | CYBA | GenBank | AB590173.1 | |
Gene (Homo sapiens) | CYBB | GenBank | NM_000397.4 | |
Gene (Homo sapiens) | p47phox | GenBank | NM_000265.7 | |
Gene (Homo sapiens) | p67phox | GenBank | LT740922.1 | |
Gene (Homo sapiens) | rac1 | GenBank | NM_006908.5 | |
Cell line (Spodoptera frugiperda) | Sf9 | Thermo Fisher Scientific | Cat. # 12659017 | RRID:CVCL_0549 |
Cell line (Homo sapiens) | FreeStyle 293F | Thermo Fisher Scientific | Cat. # R79007 | RRID:CVCL_D603 |
Transfected construct (human) | NOX2-p22 complex | This paper | Transfected construct (human) |
|
Antibody | Anti-NOX2 7D5 (Mouse monoclonal) |
MBL Life Science | Cat. # D162-3 | |
Recombinant DNA reagent | p22-GFP (plasmid) | This paper | GFP version of p22phox | |
Recombinant DNA reagent | GFP-TP1170 (plasmid) | This paper; Pleiner et al., 2018 |
PMID:29263082 |
GFP version of TP1170 |
Peptide, recombinant protein | SOD | Sigma-Aldrich | Cat. # S2515 | |
Commercial assay or kit | In-Fusion HD Cloning | Takara Bio | Cat. # 639650 | |
Chemical compound, drug | Amplex Red | GeneCopoeia | Cat. # C291 | |
Chemical compound, drug | NADPH | Cayman Chemical | Item No. 9000743 | |
Software, algorithm | SWISS-MODEL | SWISS-MODEL | RRID:SCR_013032 | |
Software, algorithm | Chimera | Chimera | RRID:SCR_004097 | |
Software, algorithm | Coot | Coot | RRID:SCR_014222 | |
Software, algorithm | Phenix | Phenix | RRID:SCR_014224 |