Figure 1. Pandemic V. cholerae strains encode a defined T6SS effector set.

(A) Left: schematic of the T6SS in its extended and contracted states in competition with a neighboring non-kin or kin cell. Right: diagrams of the core V. cholerae T6SS loci. Genes are colored by their corresponding T6SS component in the schematic to the left (white coding regions are not represented). (B) Left: V. cholerae effector types and their distribution across a hypothetical V. cholerae phylogenetic tree. The pandemic clade of V. cholerae is indicated by a black dot, and individual strains within the clade are shown in bold. Effector and immunity genes present at each T6SS locus within each strain are shown to the right. Effector-immunity pairs are colored according to their type. Extra immunity genes without a neighboring effector gene indicate orphan immunity genes. Right: All known effector types found across the three core T6SS loci. Different shapes for each locus indicate that effector-immunity pairs of the same type at different loci are distinct proteins (ex: Aux1 A = lipase, Aux2 A = pore forming toxin).