Figure 4. A-type effectors from V. anguillarum recombined into the V. cholerae Aux1 locus at least twice.

(A) Artemis alignment of the T6SS Aux1 cluster from pandemic V. cholerae strain N16961 with its potential Acc Aux1 donor (top) and Aux1 recipient (bottom) clusters, representing the transfer event indicated in Fig 3 (bottom grey box). (B) Alignment of the T6SS Aux1 cluster from V. cholerae strain EM-1676A with its potential Acc Aux1 donor (top) and Aux1 recipient (bottom), representing the transfer event indicated in Fig 3 (top grey box). Homologous regions are shown by grey boxes labeled with the nucleotide identity.