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. 2022 Dec 1;132(23):e162282. doi: 10.1172/JCI162282

Figure 7. N-specific antibodies bind to infected cells and trigger ADCC.

Figure 7

(A) Representative FACS plots showing that N-specific sera and the N-specific mAb bound to SARS-CoV-2 infected cells. 293-ACE2 cells were infected with SARS-CoV-2, and binding to N-specific antibody was assessed by flow cytometry using a secondary antibody bound to Alexa Fluor 647. Control cells were mock infected. (B) N-specific antibodies triggered ADCC. 293-ACE2 cells were infected with SARS-CoV-2 and cocultured with effector cells (NKL-mCD16 cells), together with N-specific immune sera or anti-N mAb (clone 1C7). CD107a expression on NKL-mCD16 cells was quantified by flow cytometry after 5 hours. Data are from a 1:30 dilution, from 2 experiments, each with 5 per group. All data are shown. P values were calculated using a 2-way ANOVA with Dunnett’s multiple-comparison test. Error bars represent the SEM.