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. 2022 Dec 1;132(23):e153805. doi: 10.1172/JCI153805

Figure 3. Upregulation of IDO1 in peripheral hematopoietic cell compartment mediates neuropathic pain.

Figure 3

(A) Western blotting analysis of IDO1 expression in the dorsal horn of the spinal cord with or without transcardiac perfusion with PBS after sham or SNI surgery (n = 4). (B) Time course of kynurenine levels in the serum of mice after sham (14 days) and SNI surgeries (n = 11–17 per time point). Time course of Ido1 mRNA (C) (n = 5–7 per time point) and protein (D) (n = 7 per time point) expression in the draining lymph nodes after sham or SNI surgery. (E) Time course of Ido1 mRNA expression in spleen after sham (14 days) or SNI surgeries (n = 7–8 per time point). (F) Mechanical nociceptive threshold of WT→WT, Ido1–/–→Ido1–/–, Ido1–/–→WT, and WT→Ido1–/– chimeric mice before (baseline, BL) and up to 28 days after SNI (n = 7). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham; ##P < 0.01, ###P < 0.001 versus Ido1–/– mice or perfused group by 1-way ANOVA with Bonferroni’s post hoc test (A–E) or 2-way ANOVA with Bonferroni’s post hoc test (F).