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. 2022 Dec 1;132(23):e159800. doi: 10.1172/JCI159800

Figure 7. Macrophages are activated throughout disease and express markers of M1 and M2 polarization.

Figure 7

(A) Sample correlation plot showing global transcriptomic analysis and hierarchical clustering of sciatic nerve macrophages from WT and Nmnat2V98M/R232Q mice. Each box represents 1 replicate (n = 3). (B) Volcano plot of significant codifferentially expressed (DE) genes in sciatic nerves of 2 month and 6 month Nmnat2V98M/R232Q old mice, highlighting activated macrophage markers (red) and repair SC signatures (purple). (C) GO analysis of genes enriched in sciatic nerves of 6-month-old Nmnat2V98M/R232Q mice. (D–G) Representative images of CD68, Arg1, and iNOS immunofluorescence in the sciatic nerves of (D) WT mice 3 days after nerve crush (3dpNC) and (E–G) Nmnat2V98M/R232Q sciatic nerves at 2, 6, and 12 months. Scale bars: 50 μm, 15 μm (insets). Yellow arrows indicate Arg1/CD68 colocalization and blue arrows represent Arg1/iNOS/CD68 colocalization. Inset depicts a magnified image of a polarized macrophage. All data are presented as mean ± SEM. Statistical significance determined by 1-way ANOVA with multiple comparisons.