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. Author manuscript; available in PMC: 2022 Nov 30.
Published in final edited form as: Cell Rep. 2022 Oct 18;41(3):111495. doi: 10.1016/j.celrep.2022.111495

Figure 3. miR-956 promotes EB-to-EC differentiation.

Figure 3.

(A) Schematic of the I-KCKT system.

(B and C) Midguts showing EBs marked using Su(H)-GBE-V5 (anti-V5 in green) in (B) I-KCKT-Gal4 TS, and (C) I-KCKT-Gal4TS; UAS-miR-956sp animals counterstained for all cell nuclei (DAPI in blue).

(D) Quantification of EB numbers in I-KCKT-Gal4 TS and I-KCKT-Gal4TS; UAS-miR-956.sp midguts.

(E and F) Gbe-Gal4 TS labeled EBs (green) in (E) Gbe-Gal4 TS, and (F) Gbe-Gal4 TS; UAS-miR-956sp midguts counterstained for all cell nuclei (DAPI in blue).

(G) Quantification of EB numbers in Gbe-Gal4 TS and Gbe-Gal4TS; UAS-miR-956sp midguts.

(H) Schematic of the ReDDM system.

(I and J) EC turnover analysis using (I) esgTS-ReDDM and (J) esgTS-ReDDM; UAS-miR-956sp midguts counterstained for all cell nuclei (DAPI in blue); GFP and RFP reporters in green and red, respectively.

(K) Quantification of percentage of cell turnover (red ECs/unlabeled DAPI + ECs) in esgTS-ReDDM and esgTS-ReDDM; UAS-miR-956sp midguts.

Data shown as mean ± SEM. Significance values: *p < 0.1; **p < 0.01; ****p < 0.0001. Scale bar, 25 mm. n values in the graphs indicate the number of intestines.