Table 1.
S. cerevisiae strains used in this study.
| Description | Genotype | Manufacturer |
|---|---|---|
| EG103 (SODWT) | MATa leu2-3,112 trp1-289 ura 3-52 GAL+ | Edith gralla, L angeles |
| EG118 (Sod1∆) | sod1::URA3 all other markers as EG103 | |
| EG110 (Sod2∆) | sod2::TRP1 all other markers as EG103 | |
| EG133 (Sod1∆Sod2∆) | sod1::URA3 sod2::TRP1 double mutant all other markers as EG103 | |
| EG223 (Cat1∆) | EG103, except cat1:: TRP1 | |
| EG (Sod1∆Cat1∆) | EG103, except sod1:: URA3 and cat1:: TRP1 |
The strains were cultured in YEPD medium (Yeast extract 0.5%, bacto-peptone 2% and dextrose 2%) at 28°C HYPERLINK [51]. Suspended cells were seeded in the center of a Petri dish in a continuous cycle. H2O2 (10 mM) was used as an inducer of oxidative stress and formed the positive control (PC) group. Saline 0.9% and DMSO 0.05% groups are the negative control (NC) groups.