Figure 3.

The biological effect of AYC and MCC950 on inflammasome- and pyroptosis-related molecules. (a, b) Western blotting results showing the protein levels of NLRP3, ASC, cleaved caspase-1, IL-1β, and GSDMD among the sham, OA, and AYC groups. (a, c) Levels of the above proteins among the sham, OA, and MCC950 groups. (d, e) TUNEL assay indicated that positive nuclei were increased markedly in the OA synovial tissue compared with the sham-treated group. Positive cells were reduced in the Ac-YVAD-cmk and MCC950 groups. Data in this figure are presented as mean ± SD and analyzed by parametric test. Statistical significance is determined with one-way ANOVA followed by Bonferroni's post hoc test. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.