Fig. 14.

PBAE nanoparticles functionalized with anti-CD3 enable targeted delivery of CAR or TCR mRNA for reprogramming circulating T cells in vivo, thereby effectively recognizing and eliminating tumor cells. (a) Illustrated is the preparation process of PBAE-mRNA NPs. (b) Fluorescence imaging revealed dtTomato expression in the organs of mice after intravenous injection of different dtTomato-mRNA-formulations as shown. (c) Flow cytometry analysed the percentages of different types of transfected CD45+dtTomato + cells harvested from the spleen of mice. (d) The experimental timeline and representative bioluminescent images of mice with orthotopically transplanted luciferase-LNCap C42 prostate tumor and treated with different formulations as indicated. (e) Survival curves of mice received different treatments [32]. Reproduced under the terms of the Creative Commons CC BY license. Copyright 2020, The Author(s), published by Springer Nature.