Fig. 6.

Cationic liposome/IONPs promote OVA-mRNA translation, ex vivo DC maturation, tracking DC vaccines in vivo with MRI, thereby effectively predicting anti-tumor effects. (a) Schematic illustration of the synthesis of IO-RNA-NPs, activating DCs ex vivo under magnetic field, and monitoring trained DC migration from the injection site to dLNs with an MRI-instrument. (b) Ex vivo DCs pre-treated with IO-RNA-NPs or RNA-NPs were co-cultured with OVA experienced T-cells for 48 h. IFN-gamma levels were measured by enzyme-linked immunosorbent assay (ELISA). C) Average B16F10-OVA tumor growth curves of mice received IO-RNA-NPs loaded-DC vaccine and adoptive OT-I T cells, and untreated mice. (d) Survival curves of mice grouped based on the response prediction by MRI data Reprinted with permission from Ref. [137]. Copyright 2019, American Chemical Society.