TABLE 2.
Adoptive transfer of immunity protective against primary T. cruzi infectiona
| Cells used for adoptive transfer | Mortality postchallenge (%)c |
|---|---|
| None | 15/15 (100) |
| Naive total SC | 4/19 (21)b |
| Naive CD4-depleted SC | 10/11 (91) |
| Th1 cells | 15/15 (100) |
| Th1 cells + naive CD4-depleted SC | 2/9 (22)b |
a
The indicated Th1 cell lines or cells from BALB/c mice were adoptively transferred to C.B-17 SCID mice 1 day prior to subcutaneous challenge with 1,000 T. cruzi IMT. A total of 5 × 106 cells of each cell preparation were injected per mouse. All deaths occurred within 6 weeks of infection. Mice surviving infection were still alive 3 months postchallenge. SC, spleen cells.
b
Survival significantly greater than that of groups receiving no adoptive transfer, CD4-depleted SC, and Th1 cells alone prior to T. cruzi challenge by Fisher's two-tailed exact tests (P < 0.01).
c
Number of mice that died/total number of mice in group.