Extended Data Fig. 7. Nanobody-Fc fusion proteins specific to PfPTRAMP and PfCSS inhibit parasite growth and recognize PTRAMP-CSS in merozoites.

a. Growth inhibition of parasites by D2 and H8 nanobodies and nanobody-Fc fusion proteins. Nanobodies and nanobody-Fc fusion proteins were tested at the EC₅₀ concentration for growth inhibition of P. falciparum; D2 at 283 μg/ml and D2-Fc at 1.68 mg/mL or 21.2 μM; H8 at 288 μg/mL and H8-Fc at 1.72 mg/mL or 21.7 μM. Data is shown from one independent experiment, performed in triplicate. Error bars indicate standard error of the mean. b. Recombinant PTRAMP-CSS were probed with D2-Fc or H8-Fc fusion proteins under reduced (R) and non-reduced (NR) conditions. Western blot experiment has been performed at least twice. c. PfCSS-HA merozoites were probed with D2-Fc under non-reduced conditions to detect PTRAMP-CSS. Western blot experiment was performed once.