Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 16;612(7938):123–131. doi: 10.1038/s41586-022-05440-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 4 — a, Microglial phagocytosis of aggregated Aβ is not affected by Mfge8 C2 KO in APPPS1 animals, as evidenced by FACS-based analysis of the amyloid-specific Methoxy-X04 signal (2 months: n = 4f/4m Mfge8 WT n = 5f/2m Mfge8 C2 KO; 4 months: n = 9f/3m Mfge8 WT, n = 6f/5m Mfge8 C2 KO) in microglia (identified based on CD11b^high, CD45^intermediate cells) or by b, Ultra-sensitive ELISA measurements of Aβ42 levels in purified microglia (as in a) (2 months APPPS1: n = 5f/3m Mfge8 WT, n = 3f/2m Mfge8 C2 KO; 4 months APPPS1: n = 1f/3m Mfge8 WT, n = 2f/1m Mfge8 C2 KO). c, Stereological quantification of total cortical microglia (PU.1 staining, black) or plaque-associated microglia (plaques stained with Congo Red) shows no differences in total and plaque-associated numbers of microglia in APPPS1 x Mfge8 WT versus APPPS1 x Mfge8 C2 KO animals at 2/4 months of age (for total microglia in 2-month-old animals: n = 5f/4m APPPS1 x Mfge8 WT and n = 6f/3m APPPS1 x Mfge8 C2 KO and in 4-month-old animals: n = 3f/3m APPPS1 x Mfge8 WT and 6f/2m APPPS1 x Mfge8 C2 KO; for plaque-associated microglia in 2-month-old animals: n = 5f/4m APPPS1 x Mfge8 WT and n = 6f/3m APPPS1 x Mfge8 C2 KO; in 4-month-old animals: 5f/1m APPPS1 x Mfge8 WT and 3f/3m APPPS1 x Mfge8 C2 KO). d, Cytokine levels measured in FACS-purified microglia are indistinguishable in 2- and 4-month-old APPPS1 x Mfge8 WT and APPPS1 x Mfge8 C2 KO animals APPPS1 (2 months: n = 5f/3m Mfge8 WT, n = 3f/2m Mfge8 C2 KO; 4 months: n = 3f/4m Mfge8 WT, n = 3f/2m Mfge8 C2 KO). e/f, Using staining for amyloid (hFTAA), microglia (Iba1) and dystrophic neurites (APP), the microglial plaque barrier (e) and plaque-associated neuritic dystrophy (f) were quantified in 12-month-old APP23 and 4-month-old APPPS1 x Mfge8 WT/C2 KO animals, because in these age groups, plaque load is indistinguishable between Mfge8 WT/C2 KO animals (n = 3f/4m analysed for each group), allowing for a direct comparison between genotypes. Neither measure was significantly altered in Mfge8 WT vs. C2 KO animals. Data shown are means ± S.E.M. Scale bar: 20 µm in c/e.

Source data