Table 2. The sequence of item arrangement for transferring the clones to parent and copy plates.

S.No.	Name of the item	Remarks
1	Cloning cylinders*	Place them in a fresh sterile dish
2	Forceps	Sterile, curved ones are better for holding cylinders
3	Pre-warmed trypsin	0.05% (w/v); sufficient volume
4	Pipette tip boxes	Sufficient, sterile; 1–200 μL tips for micropipette
5	Micropipette	Range 20–200 μL
6	6-well plates (sterile)	Parent plate wells can be labeled as 1P, 2P, 3P, and so on Copy plate wells can be labeled as 1C, 2C, 3C, etc.
* Cloning cylinders supplied non-sterile; these can be sterilized by placing/submerging them in a water filled glass beaker that is then autoclaved.