| Reagent | Final concentration | |
| Tris-Cl buffer (pH 6.8) | 250 mM | |
| SDS (electrophoresis grade) | 10% (w/v) | |
| Bromophenol blue | 0.5% (w/v) | |
| Glycerol | 100 mM | |
| DTT or β-ME | 10% (w/v) | |
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