Table 2.

Benefits and drawbacks of various plant pathogen detection methods.

Technique	Benefits	Drawbacks
LAMP Assays	High efficiency, specificity, simplicity and quickness; Simple equipment requirement; Constant temperature and short reaction time.	Difficulty in primer designing; Complicated process for non-specialist.
Microfluidics	Small volume of sample requirement, portable, high selectivity and fast response	External parameters such as pH, temperature and pressure can affect the analysis
MIP	Low cost, quick preparation, reproducible and stable; Resistant to high pressure, high temperature, and extreme pH conditions; Long term storage possible	Needs reduction in non-specific binding; Large-scale production is difficult
LFIA	Fast, low cost, small sample volume; Point of care diagnostics; Longer shelf life and pretreatment is not often required.	Good antibody preparation is mandatory, analysis time relies on type of sample
ddPCR	High sensitivity, absolute quantification as it needs no extrapolation	Cost of the instrument for the initial purchase is high; Requires rigorous quality control plan
CRISPR	Simple design, high efficiency	Unexpected mutations due to wrong site cleavages