Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 17;9:921829. doi: 10.3389/fcvm.2022.921829

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Zhang, Zuo, Li, Wang, Huang, Xu, Jin, Lu and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Strategy of monitoring autophagic activity in zebrafish cardiomyocytes in situ. (A) The structure of transgenic plasmid for labeling autophagosomes. (B) Microinjection of the transgenic mixture into one-cell staged embryos for generating stable lines. (C) Drug treatment of zebrafish larvae using cell culture plates. (D) Transgenic larvae are mounted, stopped heartbeat, and then imaged under a confocal microscope. (E) Confocal image shows the EGFP-positive puncta that are characterized as autophagosomes. The area of heart tissue is selected by a yellow polygon using the ImageJ software. Arabic numerals show autophagosomes. Scale bar: 20 μm. (F) The density is calculated by dividing the number of autophagosomes by the area of heart tissue.