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. 2022 Nov 17;9:921829. doi: 10.3389/fcvm.2022.921829

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Copyright © 2022 Zhang, Zuo, Li, Wang, Huang, Xu, Jin, Lu and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The density of autophagosomes in zebrafish cardiomyocytes is quantified during 2–5 dpf. (A) Representative images of zebrafish heart of Tg(myl7:EGFP-LC3) line at indicated developmental stages. Top row shows the Z-stack maximum projections (scale bar: 50 μm), and the bottom row shows the single slice (scale bar: 10 μm). Arrowhead represents the autophagosome. (B) Quantification of the density of autophagosomes in zebrafish cardiomyocytes of indicated developmental stages. The data are shown as mean ± s.e.m for each group. n = 22 at 2 dpf, n = 19 at 3 dpf, n = 38 at 4 dpf, and n = 24 at 5 dpf. ***p < 0.001. A, atrium; V, ventricle.