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. 2022 Nov 17;9:921829. doi: 10.3389/fcvm.2022.921829

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Copyright © 2022 Zhang, Zuo, Li, Wang, Huang, Xu, Jin, Lu and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The densities of autophagosomes and autolysosomes in zebrafish hearts are quantified simultaneously during 2–5 dpf. (A) Representative images of 3-dpf zebrafish heart of Tg(myl7:mRFP-EGFP-LC3) line at indicated developmental stages. The top row shows the single slice with an EGFP signal, the second row shows the single slice with an mRFP signal, and the bottom row shows the single slice with merged EGFP and mRFP signals (scale bar: 10 μm). Arrowhead represents autophagosomes, the arrow represents autolysosomes. (B) Quantification of the density of autophagosomes in zebrafish heart of Tg(myl7:mRFP-EGFP-LC3) line at indicated developmental stages. (C) Quantification of the density of autolysosomes in zebrafish heart of Tg(myl7:mRFP-EGFP-LC3) line at indicated developmental stages. The data are shown as mean ± s.e.m for each group. n = 26 at 2 dpf, n = 14 at 3 dpf, n = 18 at 4 dpf, and n = 23 at 5 dpf. *p < 0.05. A, atrium; V, ventricle.