Table 2.
Comparison of human accelerated region (HAR) massively parallel reporter assay (MPRA) studies
| Study | MPRA type |
Human cells |
Mouse cells |
Primate cells |
Statistical methods |
HARs | HGEs | Variants | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Active | DE | Total | Active | DE | Total | Active | DE | Total | ||||||
| Doan (14) | Plasmid | SH-SY5Y | Neuro2A | NT | t-test | 43% | NA | 335 | NT | NT | NT | NA | 35% | 343 rare ASD:WT |
| Girskis (23) | Plasmid | SH-SY5Y | Neuro2A | NT | t-test | 49% | 61% (H:C) | 3,132 | NT | NT | NT | NT | NT | NT |
| Weiss (72) | 5′ Lentivirus | ESC, NPC, osteoblasts | NT | NT | MPRAnalyze | NT | NT | NT | NT | NT | NT | 13% | 3% | 14,042 modern: archaic human |
| Uebbing (68) | Plasmid | NSC | NT | NT | t-test | 12% | 28% (H:C) | 1,363 | 34% | 35% | 3,027 | NA | NA | 32,776 human: nonhuman primate |
| Jagoda (31) | Plasmid | K562 | NT | NT | DESeq2 | NT | NT | NT | NT | NT | NT | 48% | 6% | 5,353 Neanderthal introgressed: not |
| Ryu (64) | 3′ Lentivirus | HS1, NPC, HS1, GPC, WTC, NPC, WTC, GPC | NT | Pt2A NPC, Pt2A GPC, Pt5C NPC, Pt5C GPC | limma | 41% | 22% (H:C) | 714 | NT | NT | NT | 28% | NA | 736 human: chimp for 7 HARs |
Abbreviations: HAR, human accelerated region; MPRA, massively parallel reporter assays; NA, not available; NT, not tested.