Table 2.
Classification of cephalosporin C acylases. Modified from Tan et al. (2018) and Pollegioni et al. (2013)
| Class | Source | Strain name | Molecular weight (kDa) | Subunit structure (kDa) | Signal peptide (aa) | Spacer (aa) | Relative activitya | |
|---|---|---|---|---|---|---|---|---|
| To GL-7-ACA | To CPC | |||||||
| I | Pseudomonas sp. | KAC-1 (130)b | 70 | 16 + 54 | 29 | 10 | 100% | 2.3% |
| Pseudomonas sp. | SY-77 (GK-16)b | 70 | 16 + 54 | 27 | 10 | 100% | 0 | |
| II | Pseudomonas sp. | A14 | 89 | 28 + 61 | 29 | - | 100% | 0 |
| III | Pseudomonas diminuta | N176 | 84 | 26 + 58 | - | 10 | 100% | 4% |
| Pseudomonas sp. | SE 83 (AcyII)c | 83 | 26 + 57 | 23 | 10 | 100% | 5% | |
| Pseudomonas diminuta | V22 | 84 | 26 + 58 | - | - | 100% | 3.2% | |
| IV | Pseudomonas sp. | SE 83 (AcyI)c | 62 | 40 + 22 | - | - | 100% | 0 |
| V | Bacillus laterospous | J1 | 70 | Monomeric | 27 | - | 100% | 0 |
aRelative activity of acylases on GL-7-ACA was taken as 100%
bThis acylase was called two different names in literature
cTwo different acylases were identified from Pseudomonas sp. SE83